Need to dig the logs to identify the problem....however, the usual
workaround for this scenarios are
can you access --> http://<mysite>/default.aspx ??
1- Place your site to Tusted Zone. via IE menu option"tools-
>internet options" . Select tab "security"--> "trusted sites"--
> "Sites" and in the new opened window add your site
("http://my_server_name") to the list.
2- Open IIS manager on the server, and the open the properties for
your virtual server. On the Home Directory tab, you probably need to
change the Application Pool the site is running under. There's
probably one named SpsAppPool or something like that. If there are
several and you're unsure, try choosing them one by one, and trying
to access the URL (the one without the default.aspx) after each
change.
-- The website may be in one application, but the _layouts, _vti_bin,
and _wpresources were in another application, try moving all into a
new application
-- Sub Virtuals (sub folders under virtual directories) might be
configured differently and when you need to make them consistent with
parent folder