Well, start with the big things, check to see if the masterpages, layout pages,
css files and any images on the home page of your portal are checked out.
Another way to tell would be to create a plain jane team site, with NO
customizations..use the v4 masterpage and then set it to anonymous access in the
site settings.
If that works, then you know you set up AA correctly and now you just need to
track down the error.